Description
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated HEF1 concentrations in Human serum, plasma and other body fluids. Using Purified Human HEF1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HEF1 and HEF1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HEF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve